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KMID : 0378019740170060811
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New Medical Journal
1974 Volume.17 No. 6 p.811 ~ p.815
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An Autoradiographic Study of Protein Synthesis and Migration in the Dorsal Lingual Mucosa of the Mice
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Abstract
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Forty-five male mice weighing approximately 20g were used in this study. They received a single intraperitoneal injection of 3pc/g of body weight of phenylalanine -3H (specific activity 3 c/mM) obtained from New England Nuclear Corporation. Mice receiving phenylalanine ´H were sacrificed at 1 hour, 4 hours, 1,4.5.7,9,11 and 14 days after injection. Dorsal tongue of all animals were immediately removed and fixed in 2% paraformaldehyde in cacodylate buffer, sectioned at 4p and stained with toluidine blue O.
The slides are dipped into Kodak NTB3 liquid emulsion and exposured for two weeks.
Following the exposure, the slides are developed for 1 minutes at 18ºC in a developer and fixed in kodak rapid fixer.
The present investigation was undertaken primarily to determine more precisely the turnover time of dorsal lingual mucosa by autoradiographic analysis.
The results were as follows.
1. Most cells in the stratum basale of the dorsal lingual. epithelium were radioactived at one day after injection.
2. Phenylalanine labeled cells were reached the lower half portion of the stratum spinosum in 4 days after injection.
3. Seven days after injection radioactive cells had moved up the upper half of stratum s0losum. 4. Tritiated phenylalanine was immediately incorporated into all differentiating epithelial layers,
entering the stratum corneum in 9 days after injection, and all labelling was gone by day 14.
5. These and similar observations may be of value in future studies of pathological processes involving
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